Ion Chromatography C18 SPE Columns For Solid Phase Extraction
C18 SPE Columns Profile
C18 (capped) SPE column was packed with octadecyl silica gel to retain nonpolar compounds through strong hydrophobic interaction. The filler can retain most organic compounds and is widely used in environment, food safety and other fields.
Technology advantage
High carbon loading, strong hydrophobicity;
Full coverage end capping reduces interference from alkaline and polar compounds;
It can be used in high pH environment.
SPE Columns Application
Detection of organic pollutants in soil, such as polycyclic aromatic hydrocarbons (PAHs);
Detection of pesticide and veterinary drug residues in food, such as antibiotics;
Analysis of pigment and sugar in food;
Desalination of aqueous solution before ion exchange.
Pretreatment Column Technical Parameter
Carbon content: 17.6%
Specific surface area: 300 m2 /g
Particle size: 40 - 60 μ m
| Filling amount/mg | Volume /ml | Package/(piece/box) |
| 500 | 3 | 50 |
| 500 | 6 | 30 |
| 1000 | 6 | 30 |
| 2000 | 12 | 30 |
| 60 | 3 | 50 |
| 1000 | 3 | 50 |
Solid phase extraction technology is based on the theory of liquid-solid chromatography. The samples are enriched, separated and purified by selective adsorption and elution. SPE is a physical extraction process including liquid phase and solid phase. In the process of solid phase extraction, the adsorption force of the adsorbent on the target compound is greater than that of the sample. When the sample passes through the SPE cartridge, the target compound is adsorbed on the surface of the solid, and other components pass through the cartridge with the sample. Finally, the target compound is eluted and collected with appropriate solvent, and then chromatographic analysis is carried out. In recent years, as a sample pretreatment technology, solid phase extraction has been more and more widely used in the laboratory.
Operation Steps
The operation is slightly different for different filler retention mechanisms (filler retention of target compounds or retention of impurities).
1. The filler retains the target compound
Solid phase extraction generally has four steps (Fig. 1)
(1) Activation - remove impurities in the cartridge, activate the filler and create a suitable solvent environment.
(2) Loading - dissolve the sample with a certain solvent, move it into the cartridge and keep the components on the cartridge.
(3) Leaching - remove interferences.
(4) Elution - elute the tested substance with a small amount of solvent and collect it.
2. Filler retention impurities
Solid phase extraction generally has three steps (Fig. 2)
(1) Activation - remove impurities in the cartridge, activate the filler and create a suitable solvent environment.
(2) Loading - move the sample into the cartridge. At this time, most of the target compounds will flow out with the sample, and the impurities will be retained on the cartridge. Therefore, this step should start to collect.
(3) Elution - elute the components with a small volume of solvent and collect them, and combine the collected solution.
Qingdao Shenghan Chromatograph Technology Co., Ltd. (SHINE) was established in 2002, specializing in the R&D, production, sales and after-sales service of ion chromatograph and relevant parts. It is a high-tech enterprise with ISO 9001 quality management system certification and ISO 24001 environmental management system certification. The total number of independent intellectual property rights, patents and software copyrights belonging to SHINE is about 100.
At present, the company has 4 series of ion chromatograph:desktop IC, portable IC, online IC and customized IC , which are widely used in environmental protection, food and drug, hydrogeology, petrochemical, health and epidemic prevention, electronic and electrical, scientific research and other industries. It basically meets the requirements of routine and trace detection of anion, cyanide, iodide, sugar, small molecular organic acid, etc. At present, it has provided perfect solutions for 5000 + users in different industries and exported to more than 40 countries and regions . In addition, SHINE is one of the few enterprises in the world that can realize mass production of IC columns.
In 2012, 2013 and 2016, SHINE has undertaken “National Major Scientific Instrument and Equipment Development Projects” for 3 times, and won the special "Science and Technology Enterprise Innovation Fund" project and the "National Key New Product Plan" project of the Ministry of Science and Technology. It is a “Individual Champion of Manufacturing Industry in Shandong”and "The Third Batch of National Manufacturing Industry Single Champion Cultivation Enterprises", and was shortlisted in “2019 Shandong High Tech Top 50 Brand” list and become “China's Top 500 Patent Enterprises”.
To protect the rights and interests of users, SHINE has set up a powerful after-sales service team with 24-hour free customer service hotline, 8 authorized after-sales service stations in China, and cooperates with foreign local agents to ensure response within 2 hour and relieve users' worries.
Through rapid development, the company has grown in enterprise scale, R & D and market share. It is a highly recognized solution provider for ion chromatography in China.
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